The ONI Discovery Kit™: dSTORM in cells imaging provides a modular workflow for immunofluorescent labeling in cultured cells, which allows you to confidently detect extra and intracellular proteins in two channels with 20 nm resolution and high sensitivity in your own samples. You provide the cells and custom antibodies, we provide the rest!
The kit enables you to:
Label targets of interest with your own primary antibodies
Use highly specific anti-mouse, rabbit, and rat secondary antibodies conjugated with best-in-class dSTORM fluorophores
Optimize key sample preparation steps, such as fixation, to preserve biological structures and avoid artifacts
Perform 2-color super-resolution dSTORM imaging in various cell types
Obtain images with 20 nm resolution using ONI’s Nanoimager
Use ONI’s CODI platform for cluster-based data analysis to extract novel nanoscale cellular information
The standard dSTORM Discovery Kit helps you prepare cells for dSTORM imaging with ONI’s optimized reagents and protocols, allowing you to save time and obtain robust super-resolution data from your targets of interest in a fast and simplified manner. We recommend this kit for researchers studying intracellular non-membrane proteins. Exceptions are structural proteins, such as tubulin and actin, which are optimally fixed with the stronger fixative.
PFA can be insufficient to cross-link membrane proteins and lead to antibody-induced protein clustering. The dSTORM Discovery Kit with strong fixative immobilizes membrane proteins using PFA and glutaraldehyde. This better preserves the native structure and enables you to confidently measure protein clustering. Not all proteins can be fixed with glutaraldehyde, as it can disrupt the epitope, so this kit provides both fixatives for you to choose the best one for your protein of interest.
